PLAT Polyclonal Antibody for Human(Homo sapiens) | EIAab.comPLAT Polyclonal Antibody for Human(Homo sapiens)

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Index > Protein center > PLAT(Gene name) > Human

PLAT (Gene name),

Tissue-type plasminogen activator (Protein name ), TPA_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

PLAT;

Protein name:

Tissue-type plasminogen activator(t-PA;t-plasminogen activator;tPA);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterodimer of chain A and chain B held by a disulfide bond. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B; binding is followed by internalization and degradation.

Function:

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

Subcellular Location:

Secreted extracellular space

Protein Attributes:

Sequence length:

562

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

562:

NYLDWIRDNM | RP

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

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Related Databases

Pfam:

PF00008

UniGene:

Hs.491582

SMR:

P00750

String:

P00750

KEGG:

hsa:5327

MIM:

173370

Uniprot:

P00750

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ELISA Kit for Human t-PA

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E0525p

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ELISA Kit for Human t-PA

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E0525h

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ELISA Kit for Human t-PA

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E0525r

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ELISA Kit for Human t-PA

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E0525m

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ELISA Kit for Human t-PA

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E0525b

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96T

CLIA Kit for Human t-PA

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U0525m

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CLIA Kit for Human t-PA

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U0525b

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96T

CLIA Kit for Human t-PA

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U0525p

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96T

CLIA Kit for Human t-PA

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U0525h

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CLIA Kit for Human t-PA

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U0525r

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Polyclonal Antibody for Human t-PA

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P0525Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human t-PA

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P0525Rb-m

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Polyclonal Antibody for Human t-PA

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P0525Rb-r

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Monoclonal Antibody for Human t-PA

Protein for Human t-PA

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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Citations

[1].

"Cocktail Blood Biomarkers: Prediction of Clinical Outcomes in Patients with Acute Ischemic Stroke"

Zeng L. · Liu J. · Wang Y. · Wang L. · Weng S. · Chen S. · Yang G.-Y.

From:European Neurology

References

1.

"Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli."
Pennica D. , Holmes W.E. , Kohr W.J. , Harkins R.N. , Vehar G.A. , Ward C.A. , Bennett W.F. , Yelverton E. , Seeburg P.H. , Heyneker H.L. , Goeddel D.V. , Collen D.
Nature301:214-221(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Melanoma.
2.

"The structure of the human tissue-type plasminogen activator gene: correlation of intron and exon structures to functional and structural domains."
Ny T. , Elgh F. , Lund B.
Proc. Natl. Acad. Sci. U.S.A.81:5355-5359(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The human tissue plasminogen activator gene."
Friezner Degen S.J. , Rajput B. , Reich E.
J. Biol. Chem.261:6972-6985(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli."
Harris T.J. , Patel T. , Marston F.A. , Little S. , Emtage J.S. , Opdenakker G. , Volckaert G. , Rombauts W. , Billiau A. , Somer P.
Mol. Biol. Med.3:279-292(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
5.

"Expression of human uterine tissue-type plasminogen activator in mouse cells using BPV vectors."
Reddy V.B. , Garramone A.J. , Sasak H. , Wei C.-M. , Watkins P. , Galli J. , Hsiung N.
DNA6:461-472(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
6.

"Nucleotide sequence of the tissue-type plasminogen activator cDNA from human fetal lung cells."
Sasaki H. , Saito Y. , Hayashi M. , Otsuka K. , Niwa M.
Nucleic Acids Res.16:5695-5695(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Fetal lung.
7.

"Variant tissue-type plasminogen activator (PLAT) cDNA obtained from human endothelial cells."
Siebert P.D. , Fong K.
Nucleic Acids Res.18:1086-1086(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
tissue: Umbilical vein.
8.

"A brain-type plasminogen activator."
Dou D.
Submitted (2000-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4)
9.

"cDNA of tissue plasminogen activator."
Liu Y. , Xu L. , Zeng Y. , He X.
Submitted (2003-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
10.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
tissue: Testis.
11.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
12.

SeattleSNPs variation discovery resource
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ASP-34; SER-136; THR-146 AND TRP-164
13.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
14.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
tissue: Brain.
tissue: Placenta.
tissue: Skin.
15.

"Isolation and characterization of the human tissue-type plasminogen activator structural gene including its 5' flanking region."
Fisher R. , Waller E.K. , Grossi G. , Thompson D. , Tizard R. , Schleuning W.-D.
J. Biol. Chem.260:11223-11230(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36
16.

"Purification and characterization of tissue plasminogen activator secreted by human embryonic lung diploid fibroblasts, IMR-90 cells."
Itagaki Y. , Yasuda H. , Morinaga T. , Mitsuda S. , Higashio K.
Agric. Biol. Chem.55:1225-1232(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-562
17.

"Purification and characterization of a melanoma cell plasminogen activator."
Wallen P. , Pohl G. , Bergsdorf N. , Raanby M. , Ny T. , Joernvall H.
Eur. J. Biochem.132:681-686(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 33-52 AND 311-330
tissue: Melanoma.
18.

"Tissue plasminogen activator: peptide analyses confirm an indirectly derived amino acid sequence, identify the active site serine residue, establish glycosylation sites, and localize variant differences."
Pohl G. , Kaellstroem M. , Bergsdorf N. , Wallen P. , Joernvall H.
Biochemistry23:3701-3707(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 36-562
tissue: Melanoma.
19.

"Isolation of cDNA sequences coding for a part of human tissue plasminogen activator."
Edlund T. , Ny T. , Raanby M. , Heden L.-O. , Palm G. , Holmgren E. , Josephson S.
Proc. Natl. Acad. Sci. U.S.A.80:349-352(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 251-358
20.

Jalah R. , Pavlakis G.N. , Felber B.J.
Submitted (2007-07) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE;SIGNAL SEQUENCE CLEAVAGE SITE
21.

"Carbohydrate structure of recombinant human uterine tissue plasminogen activator expressed in mouse epithelial cells."
Pfeiffer G. , Schmidt M. , Strube K.-H. , Geyer R.
Eur. J. Biochem.186:273-286(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
22.

"Tissue plasminogen activator has an O-linked fucose attached to threonine-61 in the epidermal growth factor domain."
Harris R.J. , Leonard C.K. , Guzzetta A.W. , Spellman M.W.
Biochemistry30:2311-2314(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-96
23.

"Disulfide pairing of the recombinant kringle-2 domain of tissue plasminogen activator produced in Escherichia coli."
Vlahos C.J. , Wilhelm O.G. , Hassell T. , Jaskunas S.R. , Bang N.U.
J. Biol. Chem.266:10070-10072(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS IN KRINGLE 2 DOMAIN
24.

"Functionally inactive protein C inhibitor in seminal plasma may be associated with infertility."
He S. , Lin Y.L. , Liu Y.X.
Mol. Hum. Reprod.5:513-519(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION;HETERODIMER WITH SERPINA5
25.

"The putative tumor suppressor LRP1B, a novel member of the low density lipoprotein (LDL) receptor family, exhibits both overlapping and distinct properties with the LDL receptor-related protein."
Liu C.-X. , Li Y. , Obermoeller-McCormick L.M. , Schwartz A.L. , Bu G.
J. Biol. Chem.276:28889-28896(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LRP1B
26.

"An unappreciated role for RNA surveillance."
Hillman R.T. , Green R.E. , Brenner S.E.
Genome Biol.5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S)
27.

"1H NMR structural characterization of a recombinant kringle 2 domain from human tissue-type plasminogen activator."
Byeon I.-J.L. , Kelley R.F. , Llinas M.
Biochemistry28:9350-9360(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF KRINGLE 2
28.

"Kringle-2 domain of the tissue-type plasminogen activator. 1H-NMR assignments and secondary structure."
Byeon I.-J.L. , Kelley R.F. , Llinas M.
Eur. J. Biochem.197:155-165(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF KRINGLE 2
29.

"Solution structure of the tissue-type plasminogen activator kringle 2 domain complexed to 6-aminohexanoic acid an antifibrinolytic drug."
Byeon I.-J.L. , Llinas M.
J. Mol. Biol.222:1035-1051(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF KRINGLE 2
30.

"Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution."
de Vos A. , Ultsch M.H. , Kelley R.F. , Padmanabhan K. , Tulinskly A. , Westbrook M.L. , Kossiakof A.A.
Biochemistry31:270-279(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF KRINGLE 2
31.

"Solution structure of the fibrin binding finger domain of tissue-type plasminogen activator determined by 1H nuclear magnetic resonance."
Downing A.K. , Driscoll P.C. , Harvey T.S. , Dudgeon T.J. , Smith B.O. , Baron M. , Campbell I.D.
J. Mol. Biol.225:821-833(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 38-85
32.

"The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator."
Smith B.O. , Downing A.K. , Driscoll P.C. , Dudgeon T.J. , Campbell I.D.
Structure3:823-833(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 36-126
33.

"The 2.3 A crystal structure of the catalytic domain of recombinant two-chain human tissue-type plasminogen activator."
Lamba D. , Bauer M. , Huber R. , Fischer S. , Rudolph R. , Kohnert U. , Bode W.
J. Mol. Biol.258:117-135(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF CATALYTIC DOMAIN
34.

"Lysine 156 promotes the anomalous proenzyme activity of tPA: X-ray crystal structure of single-chain human tPA."
Renatus M. , Engh R.A. , Stubbs M.T. , Huber R. , Fischer S. , Kohnert U. , Bode W.
EMBO J.16:4797-4805(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF CATALYTIC DOMAIN

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